What is Epitalon?

Epitalone is a synthetic peptide (a short chain of amino acids) known as Ala-Glu-Asp-Gly. It was originally derived from an extract of the pineal gland, a small organ located in the brain, and has been extensively studied for its potential health benefits, particularly as an anti-aging peptide.

Epitalon's impact on health and longevity

Epitalone is currently at the forefront of numerous health and longevity studies due to its potential impact on various aspects of human health. Epitalone appears to be a promising subject for future biomedical research, from its effects on aging to its potential role in heart health, cancer treatment, vision, neurological function, gastrointestinal health, hormonal regulation and other significant health benefits. Understanding of its true effects is still developing, existing research points to the multifaceted benefits it can offer.

Here are the potential health effects of Epitalon as described in various scientific studies.

The anti-aging potential of Epitalon

In an experiment conducted on monkeys, researchers sought to understand the effects of aging on pineal and pancreatic function, and the role Epitalone may play in mitigating these effects [1]. As the monkeys aged, the researchers observed that glucose and insulin levels increased and melatonin levels decreased at night. However, when Epitalon was administered to older monkeys, the animals showed a decrease in glucose and insulin levels and an increase in melatonin levels at night. There was also an improvement in the monkeys' ability to metabolize glucose. Epitalone had no effect on younger monkeys, suggesting that it counteracts age-related hormonal changes. This study underscores the potential of Epitalone to treat age-related metabolic and hormonal changes.

Another important study was conducted to understand the effects of Epitalon on cognitive function. In the study, Epitalone was administered to rats starting at four months of age to study its effects on learning and memory [2]. Older rats showed better cognitive function and memory. This indicates the potential utility of Epitalone as a therapeutic intervention to improve cognitive function and memory in aging populations.

Epitalone's effects on gene regulation were also evaluated in a study focusing on its effects on the structure of chromatin, the material inside a chromosome, in lymphocytes (a type of white blood cell) obtained from elderly people [3,4]. The researchers found that Epitalone activates certain genes and affects chromatin activity, suggesting a potential mechanism by which Epitalone can reverse age-related changes in gene regulation. This is another pathway through which Epitalon may exert its anti-aging effects.

In addition, there is evidence that Epitalone, along with other short biologically active peptides, can penetrate animal cells, including the cell nucleus, and interact with both DNA and RNA [5]. The peptides have been found to alter the fluorescence of some cellular compounds, suggesting specific interaction with different DNA segments. Moreover, the peptides appeared to affect specific DNA sequences, which may have implications for gene regulation and cellular function.

A series of studies have examined how Epitalone and other peptide bioregulators affect the structure of heterochromatin, a compact form of DNA, in lymphocytes obtained from elderly people [6, 7]. Studies suggest that these peptides can activate chromatin, which can potentially stimulate gene expression and improve cellular function in aging individuals.

Another study focused on Epitalone's effects on genetic stability. A study in mice showed that Epitalone significantly reduced the incidence of chromosomal aberrations, which are known to increase with age [8]. This suggests another possible avenue by which Epitalone can mitigate age-related genetic damage and potentially extend healthy life.

In addition, researchers studied the effects of different lighting conditions and substances such as Epitalon on exercise capacity and antioxidant activity in rats for two years [9]. The study showed that Epitalon can reduce the decline in physical activity with age and help restore antioxidant balance. This indicates that Epitalone has the potential to increase physical activity and maintain oxidative balance in aging populations, thereby improving quality of life.

Melatonin modulation and prevention of age-related dysregulation

A study was conducted to examine the effects of Epitalone on melatonin and cortisol production in aging monkeys [11]. Melatonin is a hormone that regulates sleep and wakefulness, while cortisol is often referred to as the "stress hormone" due to its role in the body's response to stress. As we age, the production and regulation of these hormones can become dysregulated, leading to various health problems. In the experiment, female Macaca mulatta monkeys were administered Epitalon and then monitored for melatonin and cortisol levels. The results showed that Epitalon effectively stimulated melatonin production during the evening hours, and also helped restore the normal diurnal rhythm of cortisol production in the aging monkeys. This study suggests that Epitalone may help counteract some of the age-related hormonal imbalances that occur with aging [11].

Another study examined the effects of Epitalone and a similar peptide called Vilone on melatonin production in cultures of rat pinealocytes, which are cells derived from the pineal gland, a small gland in the brain that produces melatonin [12]. The researchers observed that Epitalon positively affected the production of two key factors involved in melatonin synthesis, the enzyme AANAT and the transcription protein pCREB, resulting in increased melatonin levels. Interestingly, when Epitalon and Vilone were administered together with another hormone called norepinephrine, the expression of AANAT and pCREB was further increased, leading to even greater melatonin production [12].

In addition, the study aimed to investigate aging-induced changes in pineal gland function [13]. The researchers observed a significant decrease in melatonin levels in older monkeys, especially at night. However, when these monkeys were given Epitalone, their melatonin levels increased significantly at night, suggesting that Epitalone could potentially help alleviate some of the hormonal changes associated with aging. Other studies have shown that both Epitalone and a related peptide complex known as Epitalamine can help restore normal melatonin release in older adults and normalize their diurnal rhythms [14-16]. Such findings hold promise for potential therapeutic applications in the treatment of age-related sleep disorders and other related health issues.

Further studies investigated the potential neuroprotective effects of melatonin and Epitalone in female rats exposed to harmful environmental factors [17]. The results showed that both melatonin and Epitalone helped correct impairment of the hypothalamic-pituitary-adrenal (HPA) axis, a critical endocrine system in the body that regulates reproduction, suggesting a protective role for these substances against environmental effects on reproduction in both young and aging female rats.

The study also found that melatonin and pineal gland peptides, including Epitalone, can correct the disturbed diurnal dynamics of catecholamines, which are hormones that play a key role in regulating several bodily functions, including stress response, heart rate and blood pressure [18]. The study suggests that melatonin and Epitalone may protect the female reproductive system from the harmful effects of certain environmental toxins and show potential in maintaining reproductive health and mitigating premature aging.

These various lines of research into Epitalone's potential anti-aging effects are promising, offering insights into how it can be used in the context of age-related disorders. All of these studies also point to Epitalone's promising potential to manage various age-related changes in hormone balance and protect against environmental influences that can lead to premature aging. Conducting controlled human clinical trials is an important next step in establishing the safety and efficacy of Epitalone for potential therapeutic use in the context of human health and aging.

Epitalone and gastrointestinal benefits during aging

The peptide Epitalon (Ala-Glu-Asp-Gly) has been the subject of numerous studies on its potential benefits for gastrointestinal, pancreatic and liver health, particularly in the context of aging.

A study conducted on aged Wistar rats showed that monthly oral administration of Epitalone led to a significant increase in enzyme activity in the small intestine [19]. Enzymes are substances that help speed up chemical reactions in the body, including those related to digestion. Increased activity of these enzymes may improve the function of the small intestine, increasing its ability to absorb nutrients and its barrier function. This suggests that Epitalon has the potential to improve intestinal health, especially in the elderly.

Another study showed that Epitalon was able to increase the activity of enzymes involved in digestion in both young and aged rats [20]. The most significant improvement was observed in older rats, suggesting that Epitalone may help balance digestive function during aging by maintaining enzyme activity. Studies have also shown an interesting link between the pineal gland (a small gland in the brain) and the stomach. Peptides from the pineal gland, such as Epitalone, appear to regulate the activity of certain cells in the stomach, potentially influencing how the stomach functions and secretes hormones [21]. This underscores Epitalone's broader role in regulating gastrointestinal physiology.

In a study on rats, removal of the pineal gland led to changes in gastric and thyroid cells. These changes were reversed when the rats were given Epitalone, suggesting that it plays a role in maintaining the normal function of these cells [22]. The effect of Epitalone on enzyme activity also appears to depend on different lighting conditions and the age of the subject. The activity of a digestive enzyme called amylase was different in young and mature rats exposed to natural and constant light. Both melatonin (a hormone that regulates sleep and wakefulness cycles) and Epitalone showed a marked effect on amylase activity under different conditions, highlighting their role in regulating digestive enzymes [23].

Epitalone was able to improve glucose tolerance and insulin response, in female monkeys, which typically decline with age. This suggests that it may help maintain normal blood glucose levels and improve overall metabolic health [24]. The study also found that both Vilon and Epitalon can increase the transport of glucose and an amino acid called glycine in different regions of the small intestine in older rats. This suggests that they may improve the gut's ability to absorb important nutrients [25].

Effects on the liver antioxidant system and age-related changes

In the case of the liver, the study examined how light conditions, the hormone melatonin and a synthetic peptide called Epitalon can affect the liver's antioxidant system in rats of different ages and sexes [26]. Antioxidants help protect our cells from damage, and the liver is one of our body's key defense systems. The researchers found that the liver's antioxidant capacity remained stable at different ages and in different sexes. However, rats exposed to continuous light showed the most significant changes, suggesting that too much exposure to light could potentially harm the liver's antioxidant balance [26]. The hormone melatonin and Epitalon appeared to affect these age-related changes.

The study suggests that excessive or continuous exposure to light can disrupt the liver's antioxidant balance, potentially leading to damage. Introducing melatonin or Epitalon may help control these age-related changes, providing treatment options for age-related liver disease [26].

Antioxidant activity

Further studies investigated the antioxidant effects of pineal gland-derived peptide preparations, such as Epitalamine and Epitalon, in aged rats [27]. These peptides showed strong antioxidant effects, even stronger than melatonin, a well-known antioxidant hormone. They not only increased melatonin production, but also acted in other ways to help protect cells from damage. They appeared to stimulate the expression of antioxidant enzymes that help break down harmful substances in our bodies [27]. These findings suggest that peptides such as Epitalon may enhance our body's antioxidant defenses, potentially helping to slow the aging process.

In a related study, Epitalone injections in rats led to a reduction in lipid peroxidation, a process that can damage cell membranes, and a decrease in oxidative modification of proteins, suggesting the potent antioxidant activity of Epitalone [28].

Anti-inflammatory effect

Inflammation is the body's natural response to injury or infection. Chronic inflammation can lead to a variety of health problems. A study evaluated the effects of Epitalone, along with four other peptides, on inflammation and cell growth in human monocytic THP-1 cells, a type of white blood cell [29]. The results showed that these peptides can affect cell growth patterns and reduce inflammation. They were found to inhibit the production of certain substances, such as TNF and IL-6, which can induce inflammation. The peptides also appeared to reduce cell adhesion, a process that can contribute to inflammation. These findings suggest that peptides such as Epitalon may have anti-inflammatory properties and act beneficially in inflammatory and infectious conditions [29].

Epitalon and the body's stress and immune response

Studies have shown that Epitalone can modulate the response of cells in the hypothalamus to mild stress. The peptide appeared to affect the number of cells that produce the substance IL-2, an important role in the body's immune response. The exact mechanisms are not entirely clear, but this result suggests that Epitalone can affect stress and the body's immune response [30].

These studies provide valuable insight into Epitalone's potential liver health benefits, antioxidant properties and anti-inflammatory effects. However, further research is needed to fully understand these mechanisms and their impact on human health.

Epitalon and the immune system

Epitalone has been shown to stimulate the growth of lymphocytes in the thymus, an important organ for immune function. Studies have shown that a genetic sequence corresponding to Epitalon is present in the promoter region of the interferon-gamma gene. This suggests that Epitalone may increase the production of interferon-gamma in T cells, which helps fight disease. This becomes particularly important as we age, since our immune system tends to weaken [31].

In another experiment, scientists removed young chickens' pituitary glands, an important part of the brain that controls hormone production. The chickens began to develop various health problems, such as anemia and a weakened immune system. However, when these chickens were given Epitalon, a reversal of these problems was observed, suggesting that Epitalon could potentially help restore the health of these chickens [32]. Studies with mice have also shown that Epitalone can affect the effects of stress on the immune system. When subjected to stressful conditions, Epitalone increased the proliferation of thymocytes, a type of cell involved in the immune response. These effects correlated with changes in the activity of a specific enzyme in the brain, suggesting that Epitalone may play a role in modulating stress response and immune function [33].

Studies on birds that have had their pituitary glands removed have shown that injections of peptides, including Epitalone, can prevent thyroid atrophy and normalize immune responses and other parameters related to blood clotting and dissolution. This indicates that Epitalone has the potential to preserve thyroid integrity and function, as well as modulate immune responses and blood clotting [34]. Moreover, another study showed that Epitalone administration effectively mitigates the negative effects of pituitary dysfunction on immune function and blood clotting processes. These findings suggest that Epitalone has the potential to restore immune function and improve clotting and fibrinolysis processes in people who have undergone hypophysectomy. This potential therapy is particularly effective when administered early, such as in newborn chickens with hypophysectomy [35].

In addition, both Epitalone and a similar peptide, epitalamine, were found to affect the functional morphology of the spleen in rats (which had had their pineal glands removed). Both peptides prevented excessive growth of lymphoid cells in the spleen and increased the reduction of blood cell production outside the bone marrow. These findings suggest a regulatory effect of epithalon on immune function and overall immune status [36].

These studies suggest Epitalon's potential in strengthening our immune system and helping us stay healthy. However, further research is needed to fully understand the effects of the preaparate and confirm its benefits.

Epitalon and neurological health

Here's an overview of how epithalon can help maintain neurological health, based on the latest scientific research.

In the study [37], researchers focused on the effects of Epitalone on a type of human stem cells called gingival mesenchymal stem cells (hGMSCs). The goal of the study was to see if Epitalone could affect the development of these stem cells, specifically, whether it could encourage them to transform into nerve cells, a process known as neurogenesis. The results were promising: the researchers found that Epitalon increased the production of several key markers, including Nestin, GAP43, β Tubulin III and Doublecortin. In other words, it seemed to help stem cells develop into nerve cells.

The researchers also used molecular modeling to investigate how Epitalon can achieve these effects. Their analysis suggests that the peptide binds to specific proteins, known as histones H1/3 and H1/6, which interact with DNA. By binding to these histones, Epitalone can increase the transcription of genes involved in neuronal differentiation, ultimately leading to increased neural cell production.

In a separate set of studies [38, 39], Epitalone was administered to rats to see if it had any effect on neuronal activity in the brain. The peptide was administered intranasally, which allows it to bypass the protective barrier between the bloodstream and the brain, making it more effective. The researchers recorded spontaneous neuronal activity in the brain and found that Epitalon significantly increased this activity. In particular, they noted a two- to two-and-a-half-fold increase in the frequency of discharges between neurons. This effect appeared quickly, within minutes of administration, suggesting a direct effect of the peptide on brain cells.

These results suggest that Epitalon may have several beneficial effects on neurological health. It may help stem cells develop into nerve cells, and it may also stimulate existing nerve cells in the brain, increasing their activity. Although further research is needed, it indicates that it supports brain health and combats neurological disorders.

Epithalon and longevity

Epitalone has been studied for its potential benefits in life extension and anti-aging effects. Its potential benefits have been tested on various organisms, including fruit flies, mice and human cells, and these studies have shown significant results.

In the case of fruit flies, the researchers added Epitalone to the culture medium during the developmental stage [40]. Even at extremely low concentrations, Epitalone significantly increased the lifespan of adult flies by 11-16%. Interestingly, the increase in lifespan was not dependent on the dose of Epitalone, indicating that it was effective even at lower doses [40]. Female CBA mice were administered Epitalone subcutaneously from 6 months of age until their death [41]. Despite having no effect on body weight or food intake, Epitalone slowed age-related weight loss and slowed free radical processes, two markers commonly associated with aging. Importantly, it extended the lifespan of mice and reduced the incidence of spontaneous tumors, suggesting that it has potential anti-aging and anti-cancer effects [41].

In experiments with human fetal fibroblasts, Epitalone led to up-regulation of the catalytic subunit, an increase in telomerase enzymatic activity and telomere elongation [42, 45]. This is important because telomeres, the envelopes at the end of each DNA strand that protect our chromosomes, shorten with age. If a product like Epitalon can lengthen telomeres, it could potentially extend the lifespan of cells and even the entire body [42, 45].

In female mice, Epitalone administration did not affect food intake or body weight, but slowed the age-related decline in estrous function [43]. It also reduced the frequency of chromosomal aberrations in bone marrow cells and extended maximum lifespan by 12.3% compared to the control group. Moreover, it significantly inhibited the development of leukemia [43]. Studies on male rats exposed to different lighting regimens showed that Epitalone administration slowed the aging process, extended life expectancy and reduced the incidence of spontaneous tumors, even in the presence of unfavorable lighting conditions [44].

Moreover, in female transgenic mice carrying the HER-2/neu breast cancer gene, Epitalone administration resulted in a significant increase in the average and maximum lifespan of the mice [46]. It slowed the development of age-related reproductive disorders and inhibited tumor formation. In particular, it reduced the incidence of breast adenocarcinomas and lung metastases, showing geroprotective effects and inhibiting breast carcinogenesis [46].

In summary, these findings suggest that Epitalone has the potential to extend life and slow the aging process in many organisms. Its mechanism of action may include regulation of antioxidant processes and modulation of telomerase activity, thereby maintaining telomere length and contributing to cell longevity. It should be noted, however, that while these preliminary results are promising, further studies are needed to fully understand the long-term effects and safety of Epitalone [47] [48].

Epitalon and vision

Studies have been conducted on the potential of Epitalon peptide in the field of eye health, specifically focusing on the retina, the part of the eye responsible for collecting visual stimuli. The research cited below looked at how Epitalon peptide can help certain retinal conditions.

A group of researchers studied the potential of Epitalone to treat a condition called congenital retinal pigmentary degeneration, a genetic disorder that affects the cells responsible for vision [49]. They found that the use of Epitalone in rats led to an increase in the functional activity of their retinas while preserving their structure. Moreover, 90% rats with retinal degenerative diseases responded positively to Epitalon. The researchers believe that Epitalon may work by participating in the same processes that regulate the pineal gland (epiphysis) and the retina.

Another experiment showed that when Epitalone was administered to both pregnant rats and their offspring, the retinas of the children remained healthy significantly longer compared to those that did not receive the peptide [50]. Retinal structure and function in treated rats were preserved twice as long compared to untreated rats. Interestingly, the beneficial effects were even more pronounced when Epitalon was administered to both mothers and their children, rather than to the children themselves, suggesting that the peptide may be beneficial to retinal health even before and during pregnancy. In addition, Epitalone has been shown to promote retinal and pigment epithelial cell growth under laboratory conditions [51]. Together with another peptide called Retinalamin, Epitalon increased the proliferation of these cells, which are crucial for maintaining healthy vision. This finding underscores the potential of these peptides to stimulate the growth of retinal and epithelial cells, suggesting their potential use in therapies for eye health.

Another study also showed that Epitalon can slow the progression of hereditary retinal pigmentary degeneration, a genetic disease that leads to vision loss in rats [52]. From birth, rats receiving Epitalone had better retinal structure, higher bioelectrical activity and better overall retinal function. This underscores the potential use of Epitalone as a therapeutic agent in maintaining retinal health and function in inherited degenerative conditions.

These studies indicate that Epitalon peptide has significant potential for eye health, particularly in terms of preserving and improving retinal health and function. Further research is needed to fully understand the mechanisms behind these effects and translate these promising results into potential treatments for individuals.

Antitumor potential of Epitalon

Epitalone has been the subject of various studies regarding its potential anticancer activity. Below, we will discuss several study results, all of which suggest that Epitalone may be a promising candidate for future anticancer therapies [53-60].

In a study, researchers found that Epitalone can limit the growth of colon tumors in rats. The researchers observed that the peptide reduces cell proliferation, or in other words, slows down the division and growth of cancer cells. Epitalone not only reduced the size of the tumors, but also increased cell death (apoptosis) within the tumors. Interestingly, this positive effect was most pronounced when Epitalone was administered throughout the experiment, suggesting that regular, long-term use of the peptide may be the most beneficial approach [53].

In another experiment, researchers studied the effect of Epitalone on spontaneous tumor formation (the process by which normal cells transform into cancer cells) in mice. They discovered that regular administration of small doses of Epitalone could significantly reduce the number of mice that develop malignant tumors and even prevent metastasis (cancer that has spread from its original location to another part of the body) [54]. Moreover, in one study, male rats were treated with Epitalone and a carcinogen called 1,2-dimethylhydrazine (DMH) to induce colon cancer [55]. The study showed that Epitalon significantly reduced the number of colon tumors in the rats and even reduced the size of the tumors. Interestingly, Epitalone also seemed to reduce the number of tumors in other parts of the intestine, such as the jejunum and ileum [55].

One study also analyzed the effect of Epitalone on the development of spontaneous breast tumors in HER-2/neu transgenic mice, a model commonly used in breast cancer research [56]. Epitalone treatment resulted in a reduction in both the number and size of tumors compared to the control group. In addition, the formulation reduced the expression of the HER-2/neu gene, which is often associated with breast cancer [56]. The inhibitory effect of Epitalone was also observed in a study involving erbB-2/neu transgenic mice, another breast cancer research model [57]. The results showed that Epitalone administration resulted in a lower incidence of multiple tumors per animal and reduced the size of mammary adenocarcinoma (a type of breast cancer) compared to the control group [57].

Epitalone was also found to slow the growth of a specific type of sarcoma in rats. However, unlike other studies, the researchers found that the peptide did not act directly on the cancer cells. Instead, it seemed to work by affecting blood flow to the tumor, which led to increased cell death within the tumor [58]. In addition, a comprehensive review indicated that Epitalone could be used in the prevention of breast cancer. Various studies have shown that the peptide can inhibit the development of breast cancer in rodents, potentially offering a new preventative measure for women [59].

Moreover, the study analyzed genome stability in people with ductal breast cancer (DBC). DBC, a common type of breast cancer, is associated with high genome instability and unique changes in gene activity. The use of Epitalone and nickel ions has shown a protective effect on DBC cell cultures. It is suggested that these two substances can potentially protect against genome instability that can lead to cancer development. This protective effect indicates that Epitalone could potentially be used in combination with other treatments to enhance their efficacy [60].

Thus, while these findings need to be confirmed in further studies, particularly in human trials, they provide fascinating insights into Epitalone's potential as an anticancer and anti-tumor agent. Its ability to inhibit cell proliferation, promote cell death in tumors and potentially protect against genetic instability could make it a valuable tool in the fight against cancer.

Epitalon vs. thyroid hormone

Several studies have looked at the effects of Epitalon on thyroid health and hormonal status.

In the study, the researchers looked at rats living in different types of light [61]. They found that when the rats lived in constant light, they had more of two important thyroid hormones in their blood. However, if the rats lived in darkness, they had fewer of these hormones. Interestingly, when the rats lived in natural light, which changes with the seasons, the levels of these hormones also changed - they were lowest in autumn and highest in spring. The researchers also found that hormone levels changed with age in rats, but that administration of Epitalone and melatonin could slow these changes [61]. The findings suggest that Epitalone and melatonin may be able to help maintain normal thyroid function with age and under different light conditions.

Moreover, in another study, researchers removed the pituitary gland from chickens, which caused their thyroid gland to function poorly and their hormone levels to be unstable [62, 63]. When the chickens were given Epitalon for 40 days, the structure of their thyroid glands improved and their hormone levels began to balance again. Interestingly, this effect was more pronounced in younger chickens, suggesting that the efficacy of these peptides may be influenced by age [62, 63].

Another study examined the effects of Epitalon peptide on the thyroid gland in mature and older birds [64]. The researchers found that these peptides could prevent thyroid damage caused by removal of the pituitary gland. It is noteworthy that the restoration of thyroid function was more pronounced in younger chickens. These findings suggest that Epitalon peptide administration may be an effective way to protect against thyroid problems and maintain a healthy thyroid balance [64].

Together, these studies suggest that Epitalon shows potential benefits for thyroid health. However, further research is needed to fully understand how it works and how it can be used therapeutically.

Other potential health effects of Epitalon

Epitalone is attracting increasing attention in the field of medical research due to its potential health benefits. Its effects on various physiological processes and pathological conditions have been studied. From protective effects on the kidneys to modulation of gene expression in the brain, the benefits of Epitalone appear to cover a wide range of health implications. These include:

Protection of kidneys damaged by rhabdomyolysis

A study focused on understanding the effects of Epitalone on kidneys damaged by rhabdomyolysis found that this tetrapeptide has the potential to provide significant renal protection [65]. Rhabdomyolysis can lead to kidney damage through toxic damage to renal cells, oxidative stress and imbalance of energy metabolism. The introduction of Epitalon has helped counteract these damaging mechanisms, thus helping to maintain kidney function and prevent acute renal failure.

Epitalone reduces chromosomal instability in patients with hypertrophic cardiomyopathy

Analysis of functional genome markers in individuals with hypertrophic cardiomyopathy (HCM) and their family members showed an increased incidence of spontaneous quantitative and structural abnormalities [66]. It has been observed that Epitalon, a peptide bioregulator, exerts a protective effect by reducing the aforementioned chromosomal instability. This means that Epitalone could potentially serve as a preventive strategy for individuals at increased risk of developing hypertrophic cardiomyopathy.

Epitalone's role in modulating gene expression in the brain

Studies using the microchip technique to examine the effects of Epitalone on gene expression in mouse brains have shown significant changes in the expression of 53 transcripts following Epitalone administration [67]. Epitalone appears to modulate the expression of genes related to important physiological processes, such as the cell cycle, apoptosis and biosynthesis. This indicates that Epitalone may have a potential role in modulating cellular processes and exerting tissue-specific biological effects.

Effect of Epitalon on digestive enzyme activity

A study in rats showed that Epitalone can affect the activity of proteolytic digestive enzymes in the pancreas and gastric mucosa during aging [68]. Epitalone restored normal patterns of enzyme activity, especially pepsin, in rats subjected to continuous illumination - a condition that disrupted the standard age-related dynamics of enzyme activity.

Regulation of apoptosis and necrosis by short regulatory peptides, including Epitalone

Several in vitro experiments have been conducted to understand the effects of Epitalone and other short peptides on biological processes such as apoptosis and necrosis [69] [70]. These peptides have shown the ability to reduce lipid peroxidation, increase the stability of red blood cell membranes and regulate intracellular reactive oxygen species. Epitalone has shown potential in inhibiting programmed cell death processes, suggesting potential therapeutic applications in various physiological and pathological contexts.

Epitalone delays oocyte aging by modulating mitochondrial activity and ROS levels

A study examining the effects of Epitalone on aging oocytes after ovulation found that Epitalone effectively reduced intracellular reactive oxygen species (ROS) and mitigated other aging-related damage [71]. By increasing mitochondrial membrane potential and modulating ROS levels, Epitalone appears to delay oocyte aging, suggesting potential applications in the treatment of infertility.

Epitalone modulates pineal gland secretion under stress conditions

Epitalone was found to selectively affect pineal gland secretion under stress conditions, preventing structural changes in the pineal parenchyma [72]. These findings suggest that Epitalone may be a potential therapeutic agent for treating stress-related disorders and promoting overall well-being by regulating pineal gland function.

Methods of administration of Epitalon

Epitalone, a synthetic peptide, can be delivered to the body in several different ways. Here are the main forms of Epitalone administration:

1. Oral form: Epitalon is available in the form of capsules designed to be taken orally. This method is simple, as the capsules are taken just like any other supplement or medication. The dosage is usually indicated on the package, which must be strictly followed.
2. Nasal spray: This formulation provides an alternative method of administration for people who do not want to swallow capsules or do not like injections. The nasal spray delivers the peptide directly into the bloodstream through the nasal tissues. This method may be more convenient for some users and allows easy control of dosage.
3. Injection mold: The most common and probably most effective form of Epitalone administration is by injection. The peptide is often sold in powdered form, which requires reconstitution before administration. This usually involves adding bacteriostatic water to the vial containing the peptide powder. Once properly mixed, the solution is ready for injection.

It is important to remember that there are several types of injections, and the best method may depend on personal comfort and the specific use case:

• Subcutaneous injection: This type of injection involves inserting a needle just under the skin. It is commonly performed in the abdominal area, but can also be performed in other fatty areas of the body.
• Intramuscular injection: With this method, the needle is inserted deeper into the body to reach the muscle tissue. This method may allow for faster absorption compared to a subcutaneous injection.

Epitalone has a systemic effect, that is, once it enters the bloodstream it affects the entire body. Different methods of administration have different efficacy in terms of absorption. Most epithalon enters the bloodstream by injection. An interesting and very good route of administration is through the nasal mucosa in a saline solution with 3% DMSO, which greatly increases the absorption of the peptide.

Epitalon dosage

Based on the available scientific studies, Epitalon was typically administered at a dose of 10 mg of Epitalon injected subcutaneously three times a week for three weeks, and this cycle is to be repeated once a year.

According to another method, 10 mg of Epitalone should be injected intramuscularly every day for 10 consecutive days. This regimen should be used annually for a total of two years.

In another approach, 10 mg of Epitalone is injected intramuscularly every third day until a cumulative dose of 50 mg is reached. This process is to be carried out twice a year for three years.

A separate regimen involves injecting 1 mg of Epitalone subcutaneously every night. Alternatively, 1.5 mg intranasally every day for 30 days.

However, in some applications, it has been found that the optimal dose range can be between 5 and 10 mg per day. It is usually recommended to start with a lower dose, which can be gradually increased over time as adaptation occurs.

Side effects of Epitalon

No significant adverse reactions have been reported in clinical or experimental studies of Epitalon. However, always monitor for any adverse reactions or interactions when taking a new supplement, or formulation. Some reported side effects include injection site reactions such as pain, redness or swelling.

Always consult your doctor before starting a new treatment regimen.

Evaluation of Epitalon based on the above scientific studies

Epitalone, also known as Epithalon or Epithalone, is a synthetic tetrapeptide, meaning that it is composed of four amino acids: Ala-Glu-Asp-Gly. This peptide has attracted considerable interest from the scientific community due to its potential impact on various aspects of human health and longevity. Although understanding of this peptide is still evolving, early studies and trials have shown promising results, particularly in aging, cardiovascular health, neuronal function, vision, cancer, stress, and thyroid and endocrine function.

The anti-aging effect of Epitalon is primarily attributed to its effects on telomere length and telomerase activity. Telomeres, the protective sheaths at the ends of chromosomes, shorten with each cell division, leading to cellular aging. Epitalone has been shown to promote telomerase activity, thereby helping to maintain telomere length and potentially extending cell lifespan. This could have far-reaching implications for age-related diseases and overall longevity. In addition, Epitalone appears to have a significant effect on the aging process by interacting with the pineal gland and the resulting regulation of melatonin production. In various studies, it has shown potential in slowing age-related degradation, demonstrating the ability to extend the active lifespan of animals.

The potential benefits of Epitalon go beyond its anti-aging effects. Studies suggest that Epitalon may have beneficial effects on the cardiovascular system, particularly on vascular endothelial health. By modulating the expression of vascular endothelial growth factor (VEGF), Epitalon may promote angiogenesis, the formation of new blood vessels, thereby improving cardiovascular function. In addition, it has shown promise in reducing lipid peroxidation and clot formation, key factors in cardiovascular disease. These studies suggest a possible protective role for Epitalone in maintaining cardiovascular health and preventing conditions such as heart disease.

With regard to cancer, some studies suggest that Epitalon may potentially offer protective effects, at least in certain contexts. For example, a study on ductal breast cancer found that Epitalone can inhibit the development and growth of malignant cells, suggesting a potential role in combination therapy for this form of cancer. However, further studies are needed to determine any therapeutic uses of Epitalone in cancer treatment.

Importantly, Epitalon appears to have a significant effect on the endocrine system, particularly the thyroid gland. Studies on animals of various ages have shown that Epitalon can help regulate thyroid function and thyroid hormone levels. What's more, some peptides engineered from pituitary amino acids have shown the potential to restore normal thyroid function in hypophysectomized chickens. These studies indicate the potential of Epitalone as a therapeutic intervention to maintain thyroid function, although again, further studies are needed to confirm these findings.

While preliminary studies provide insight into the potential benefits of Epitalone, it should be noted that our understanding of this peptide and its effects is still at an early stage. As with any bioactive compound, the use of Epitalone should be approached with caution until more robust data are available. Many of the studies conducted to date have involved animals. However, human studies are needed to fully appreciate the therapeutic potential and safety profile of Epitalone.

Potential age-related differences in physiological responses to Epitalon also suggest that the peptide's effectiveness may vary across age groups. Therefore, more extensive human studies are crucial before we can establish with certainty the role of Epitalone in supporting health.

In conclusion, Epitalon represents a fascinating preparation with significant potential in anti-aging, cardiovascular health, cancer treatment and regulation of thyroid function. However, more in-depth and extensive studies are needed to fully realize this potential in a clinical setting.

Disclaimer

This article was written for educational purposes and is intended to raise awareness of the substance being discussed. It is important to note that the substance discussed is a substance, not a specific product. The information contained in the text is based on available scientific research and is not intended to serve as medical advice or promote self-medication. The reader should consult any health and treatment decisions with a qualified health professional.

Sources:

1. Goncharova, N. D., Vengerin, A. A., Khavinson, V. K.h, & Lapin, B. A. (2005). Pineal peptides restore the age-related disturbances in hormonal functions of the pineal gland and the pancreas. Experimental gerontology, 40(1-2), 51-57. https://doi.org/10.1016/j.exger.2004.10.004 https://pubmed.ncbi.nlm.nih.gov/15664732/
2. Vinogradova I. A. (2006). Eksperimental'naia i klinicheskaia farmakologiia, 69(6), 13-16. https://pubmed.ncbi.nlm.nih.gov/17209456/
3. Khavinson, V. K.h, Lezhava, T. A., Monaselidze, J. R., Yokhadze, T. A., Dvalishvili, N. A., Bablishvili, N. K., & Trofimova, S. V. (2003). Epitalon peptide activates chromatin at the old age. Neuro endocrinology letters, 24(5), 329-333. https://pubmed.ncbi.nlm.nih.gov/14647006/
4. Anisimov, S. V., Bokheler, K. R., Khavinson, V. K.h, & Anisimov, V. N. (2002). Studies of the effects of Vilon and Epithalon on gene expression in mouse heart using DNA-microarray technology. Bulletin of experimental biology and medicine, 133(3), 293-299. https://doi.org/10.1023/a:1015859322630 https://pubmed.ncbi.nlm.nih.gov/12360356/
5. Fedoreyeva, L. I., Kireev, I. I., Khavinson, V. K.h, & Vanyushin, B. F. (2011). Penetration of short fluorescence-labeled peptides into the nucleus in HeLa cells and in vitro specific interaction of the peptides with deoxyribooligonucleotides and DNA. Biochemistry. Biokhimiia, 76(11), 1210-1219. https://doi.org/10.1134/S0006297911110022 https://pubmed.ncbi.nlm.nih.gov/22117547/
6. Lezhava, T., Monaselidze, J., Kadotani, T., Dvalishvili, N., & Buadze, T. (2006). Anti-aging peptide bioregulators induce reactivation of chromatin. Georgian medical news, (133), 111-115. https://pubmed.ncbi.nlm.nih.gov/16705247/
7. Lezhava, T., Yokhadze, T., Monaselidze, J., Buadze, T., Gaiozishvili, M., Sigua, T., Khujadze, I., Gogidze, K., Mikaia, N., & Chigvinadze, N. (2023). EPIGENETIC MODIFICATION UNDER THE INFLUENCE OF PEPTIDE BIOREGULATORS ON THE "OLD" CHROMATIN. Georgian medical news, (335), 79-83. https://pubmed.ncbi.nlm.nih.gov/37042594/
8. Rosenfeld, S. V., Togo, E. F., Mikheev, V. S., Popovich, I. G., Khavinson, V. K.h, & Anisimov, V. N. (2002). Effect of epithalon on the incidence of chromosome aberrations in senescence-accelerated mice. Bulletin of experimental biology and medicine, 133(3), 274-276. https://doi.org/10.1023/a:1015899003974 https://pubmed.ncbi.nlm.nih.gov/12360351/
9. Vingradova, I. A., Iliukha, V. A., Fedorova, A. S., Khizhkin, E. A., Unzhakov, A. R., & Iunash, V. D. (2007). Advances in gerontology = Uspekhi gerontologii, 20(1), 66-73. https://pubmed.ncbi.nlm.nih.gov/17969589/
10. Ivko, O. M., Drobintseva, A. O., Leont'eva, D. O., Kvetnoy, I. M., Polyakova, V. O., & Linkova, N. S. (2020). Advances in gerontology = Uspekhi gerontologii, 33(4), 741-747. https://pubmed.ncbi.nlm.nih.gov/33342107/
11. Khavinson, V., Goncharova, N., & Lapin, B. (2001). Synthetic tetrapeptide epithelium restores disturbed neuroendocrine regulation in senescent monkeys. Neuro endocrinology letters, 22(4), 251-254. https://pubmed.ncbi.nlm.nih.gov/11524632/
12. Khavinson, V. K.h, Linkova, N. S., Kvetnoy, I. M., Kvetnaia, T. V., Polyakova, V. O., & Korf, H. W. (2012). Molecular cellular mechanisms of peptide regulation of melatonin synthesis in pinealocyte culture. Bulletin of experimental biology and medicine, 153(2), 255-258. https://doi.org/10.1007/s10517-012-1689-5 https://pubmed.ncbi.nlm.nih.gov/22816096/
13. Goncharova, N. D., Vengerin, A. A., Shmaliĭ, A. V., & Khavinson, V. K.h (2003). Peptidnaia korrektsiia vozrastnykh narusheniĭ funktsii épifiza u obez'ian [Peptide correction of age-related pineal disturbances in monkeys]. Advances in gerontology = Uspekhi gerontologii, 12, 121-127. https://pubmed.ncbi.nlm.nih.gov/14743609/
14. Korkushko, O. V., Lapin, B. A., Goncharova, N. D., Khavinson, V. K.h, Shatilo, V. B., Vengerin, A. A., Antoniuk-Shcheglova, I. A., & Magdich, L. V.. (2007). Advances in gerontology = Uspekhi gerontologii, 20(1), 74-85. https://pubmed.ncbi.nlm.nih.gov/17969590/
15. Khavinson, V. K., Yakovleva, N. D., Popuchiev, V. V., Kvetnoi, I. M., & Manokhina, R. P. (2001). Reparative effect of epithalon on pineal gland ultrastructure in gamma-irradiated rats. Bulletin of experimental biology and medicine, 131(1), 81-85. https://doi.org/10.1023/a:1017599100641 https://pubmed.ncbi.nlm.nih.gov/11329090/
16. Goncharova, N. D., Khavinson, B. K., & Lapin, B. A. (2001). Regulatory effect of Epithalon on production of melatonin and cortisol in old monkeys. Bulletin of experimental biology and medicine, 131(4), 394-396. https://doi.org/10.1023/a:1017928925177 https://pubmed.ncbi.nlm.nih.gov/11550036/
17. Korenevsky, A. V., Milyutina, Y. P., Bukalyov, A. V., Baranova, Y. P., Vinogradova, I. A., & Arutjunyan, A. V. (2013). Advances in gerontology = Uspekhi gerontologii, 26(2), 263-274. https://pubmed.ncbi.nlm.nih.gov/28976150/
18. Arutjunyan, A., Kozina, L., Milyutina, Y., Korenevsky, A., Stepanov, M., & Arutyunov, V. (2012). Melatonin and pineal gland peptides are able to correct the impairment of reproductive cycles in rats. Current aging science, 5(3), 178-185. https://doi.org/10.2174/1874609811205030003 https://pubmed.ncbi.nlm.nih.gov/23237594/
19. Khavinson, V. K.h, Timofeeva, N. M., Malinin, V. V., Gordova, L. A., & Nikitina, A. A. (2002). Effect of vilon and epithalon on activity of enzymes in epithelial and subepithelial layers in small intestine of old rats. Bulletin of experimental biology and medicine, 134(6), 562-564. https://doi.org/10.1023/a:1022913228900 https://pubmed.ncbi.nlm.nih.gov/12660839/
20. Khavinson, V. K.h, Malinin, V. V., Timofeeva, N. M., Egorova, V. V., & Nikitina, A. A. (2002). Effects of epithalon on activities of gastrointestinal enzymes in young and old rats. Bulletin of experimental biology and medicine, 133(3), 290-292. https://doi.org/10.1023/a:1015807305791 https://pubmed.ncbi.nlm.nih.gov/12360355/
21. Khavinson, V. K., Popuchiev, V. V., Kvetnoii, I. M., Yuzhakov, V. V., & Kotlova, L. N. (2000). Regulating effect of epithalone on gastric endocrine cells in pinealectomized rats. Bulletin of experimental biology and medicine, 130(12), 1169-1171. https://pubmed.ncbi.nlm.nih.gov/11276313/
22. Khavinson, V. K.h, Kvetnoĭ, I. M., Popuchiev, V. V., Iuzhakov, V. V., & Kotlova, L. N. (2001). Vliianie peptidov pineal'noĭ zhelezy na neĭroéndokrinnye vzaimosviazi posle pinealéktomii [Effect of pineal peptides on neuroendocrine system after pinealectomy]. Arkhiv pathologii, 63(3), 18-21. https://pubmed.ncbi.nlm.nih.gov/11452647/
23. Svechkina, E. B., Tiutiunnik, N. N., & Vinogradova, I. A. (2006). Advances in gerontology = Uspekhi gerontologii, 19, 66-71. https://pubmed.ncbi.nlm.nih.gov/17152723/
24. Goncharova, N. D., Vengerin, A. A., Oganyan, T. E., & Lapin, B. A. (2004). Age-associated changes in hormonal function of the pancreas and regulation of blood glucose in monkeys. Bulletin of experimental biology and medicine, 137(3), 280-283. https://doi.org/10.1023/b:bebm.0000031570.81043.f9 https://pubmed.ncbi.nlm.nih.gov/15232640/
25. Khavinson, V. K.h, Egorova, V. V., Timofeeva, N. M., Malinin, V. V., Gordova, L. A., & Gromova, L. V.. (2002). Effect of Vilon and Epithalon on glucose and glycine absorption in various regions of small intestine in aged rats. Bulletin of experimental biology and medicine, 133(5), 494-496. https://doi.org/10.1023/a:1019878224754 https://pubmed.ncbi.nlm.nih.gov/12420071/
26. Il'ina, T. N., Vinogradova, I. A., Iliukha, V. A., Khizhkin, E. A., Anisimov, V. N., & Khavinson, V. K.h (2008). Advances in gerontology = Uspekhi gerontologii, 21(3), 386-393. https://pubmed.ncbi.nlm.nih.gov/19432171/
27. Kozina, L. S., Arutjunyan, A. V., & Khavinson, V. K.h (2007). Antioxidant properties of geroprotective peptides of the pineal gland. Archives of gerontology and geriatrics, 44 Suppl 1, 213-216. https://doi.org/10.1016/j.archger.2007.01.029 https://pubmed.ncbi.nlm.nih.gov/17317455/
28. Kozina L. S. (2007). Effects of bioactive tetrapeptides on free-radical processes. Bulletin of experimental biology and medicine, 143(6), 744-746. https://doi.org/10.1007/s10517-007-0230-8 https://pubmed.ncbi.nlm.nih.gov/18239817/
29. Avolio, F., Martinotti, S., Khavinson, V. K., Esposito, J. E., Giambuzzi, G., Marino, A., Mironova, E., Pulcini, R., Robuffo, I., Bologna, G., Simeone, P., Lanuti, P., Guarnieri, S., Trofimova, S., Procopio, A. D., & Toniato, E. (2022). Peptides Regulating Proliferative Activity and Inflammatory Pathways in the Monocyte/Macrophage THP-1 Cell Line. International journal of molecular sciences, 23(7), 3607. https://doi.org/10.3390/ijms23073607 https://pubmed.ncbi.nlm.nih.gov/35408963/
30. Barabanova, S. V., Artyukhina, Z. E., Kazakova, T. B., Khavinson, V. K.h, Malinin, V. V., & Korneva, E. A. (2006). Interleukin-2 concentration in hypothalamic structures of rats receiving peptides during mild stress. Bulletin of experimental biology and medicine, 141(4), 390-393. https://doi.org/10.1007/s10517-006-0179-z https://pubmed.ncbi.nlm.nih.gov/17152351/
31. Lin'kova, N. S., Kuznik, B. I., & Khavinson, V. K.h (2012). Advances in gerontology = Uspekhi of gerontology, 25(3), 478-482. https://pubmed.ncbi.nlm.nih.gov/23289226/
32. Kuznik, B. I., Pateiuk, A. V., Baranchugova, L. M., & Rusaeva, N. S. (2008). Advances in gerontology = Uspekhi gerontologii, 21(3), 372-381. https://pubmed.ncbi.nlm.nih.gov/19432169/
33. Khavinson, V. K.h, Korneva, E. A., Malinin, V. V., Rybakina, E. G., Pivanovich, I. Y., & Shanin, S. N. (2002). Effect of epithalon on interleukin-1beta signal transduction and the reaction of thymocyte blast transformation under stress. Neuro endocrinology letters, 23(5-6), 411-416. https://pubmed.ncbi.nlm.nih.gov/12500162/
34. Kuznik, B. I., Pateiuk, A. V., Rusaeva, N. S., Baranchugova, L. M., & Obydenko, V. I. (2010). Pathologicheskaia fiziologiia i eksperimental'naia terapiia, (1), 14-18. https://pubmed.ncbi.nlm.nih.gov/20731122/
35. Kuznik, B. I., Pateiuk, A. V., Khavinson, V. K.h, & Malinin, V. V. (2004). Vliianie épitalona na immunitet i gemostaz u gipofizéktomocheskikh tsypliat i starykh kur [Effect of epitalon on the immunity and hemostasis in hypophysectomized chicken and old hens]. Advances in gerontology = Uspekhi gerontologii, 13, 90-93. https://pubmed.ncbi.nlm.nih.gov/15490730/
36. Khavinson, V. K., Konovalov, S. S., Yuzhakov, V. V., Popuchiev, V. V., & Kvetnoi, I. M. (2001). Modulating effects of epithalamin and epithalon on the functional morphology of the spleen in old pinealectomized rats. Bulletin of experimental biology and medicine, 132(5), 1116-1120. https://doi.org/10.1023/a:1017989113287 https://pubmed.ncbi.nlm.nih.gov/11865335/
37. Khavinson, V., Diomede, F., Mironova, E., Linkova, N., Trofimova, S., Trubiani, O., Caputi, S., & Sinjari, B. (2020). AEDG Peptide (Epitalon) Stimulates Gene Expression and Protein Synthesis during Neurogenesis: Possible Epigenetic Mechanism. Molecules (Basel, Switzerland), 25(3), 609. https://doi.org/10.3390/molecules25030609 https://pubmed.ncbi.nlm.nih.gov/32019204/
38. Sibarov, D. A., Vol'nova, A. B., Frolov, D. S., & Nozdrachev, A. D. (2007). Effects of intranasal administration of epithalon on neural activity in the rat neocortex. Neuroscience and behavioral physiology, 37(9), 889-893. https://doi.org/10.1007/s11055-007-0095-3 https://pubmed.ncbi.nlm.nih.gov/17955380/
39. Sibarov, D. A., Vol'nova, A. B., Frolov, D. S., & Nosdrachev, A. D. (2006). Rossiiskii fiziologicheskii zhurnal imeni I.M. Sechenova, 92(8), 949-956. https://pubmed.ncbi.nlm.nih.gov/17217245/
40. Khavinson, V. K., Izmaylov, D. M., Obukhova, L. K., & Malinin, V. V.. (2000). Effect of epithalon on the lifespan increase in Drosophila melanogaster. Mechanisms of aging and development, 120(1-3), 141-149. https://doi.org/10.1016/s0047-6374(00)00217-7 https://pubmed.ncbi.nlm.nih.gov/11087911/
41. Anisimov, V. N., Khavinson, V. K.h, Zavarzina, N. I.u, Zabezhinskiĭ, M. A., Zimina, O. A., Popovich, I. G., Shtylik, A. V., Arutiunian, A. V., Oparina, T. I., & Prokopenko, V. M. (2001). Vliianie peptida épifiza na pokazateli biologicheskogo vozrasta i prodolzhitel'nost' zhizni mysheĭ [Effect of pineal peptide on parameters of the biological age and life span in mice]. Rossiiskii fiziologicheskii zhurnal imeni I.M. Sechenova, 87(1), 125-136. https://pubmed.ncbi.nlm.nih.gov/11227856/
42. Khavinson, V. K.h, Bondarev, I. E., & Butyugov, A. A. (2003). Epithalon peptide induces telomerase activity and telomere elongation in human somatic cells. Bulletin of experimental biology and medicine, 135(6), 590-592. https://doi.org/10.1023/a:1025493705728 https://pubmed.ncbi.nlm.nih.gov/12937682/
43. Anisimov, V. N., Khavinson, V. K.h, Popovich, I. G., Zabezhinski, M. A., Alimova, I. N., Rosenfeld, S. V., Zavarzina, N. Y., Semenchenko, A. V., & Yashin, A. I. (2003). Effect of Epitalon on biomarkers of aging, life span and spontaneous tumor incidence in female Swiss-derived SHR mice. Biogerontology, 4(4), 193-202. https://doi.org/10.1023/a:1025114230714 https://pubmed.ncbi.nlm.nih.gov/14501183/
44. Vinogradova, I. A., Bukalev, A. V., Zabezhinski, M. A., Semenchenko, A. V., Khavinson, V. K.h, & Anisimov, V. N. (2008). Geroprotective effect of ala-glu-asp-gly peptide in male rats exposed to different illumination regimens. Bulletin of experimental biology and medicine, 145(4), 472-477. https://doi.org/10.1007/s10517-008-0121-7 https://pubmed.ncbi.nlm.nih.gov/19110597/
45. Khavinson, V. K.h, Bondarev, I. E., Butyugov, A. A., & Smirnova, T. D. (2004). Peptide promotes overcoming of the division limit in human somatic cell. Bulletin of experimental biology and medicine, 137(5), 503-506. https://doi.org/10.1023/b:bebm.0000038164.49947.8c https://pubmed.ncbi.nlm.nih.gov/15455129/
46. Anisimov, V. N., Khavinson, V. K.h, Alimova, I. N., Semchenko, A. V., & Yashin, A. I. (2002). Epithalon decelerates aging and suppresses development of breast adenocarcinomas in transgenic her-2/neu mice. Bulletin of experimental biology and medicine, 134(2), 187-190. https://doi.org/10.1023/a:1021104819170 https://pubmed.ncbi.nlm.nih.gov/12459848/
47. Khavinson V. K.h (2002). Peptides and Ageing. Neuro endocrinology letters, 23 Suppl 3, 11-144. https://pubmed.ncbi.nlm.nih.gov/12374906/
48. Khavinson, V. K.h, & Golubev, A. G. (2002). Starenie épifiza [Aging of the pineal gland]. Advances in gerontology = Uspekhi gerontologii, 9, 67-72. https://pubmed.ncbi.nlm.nih.gov/12096440/
49. Khavinson, V., Razumovsky, M., Trofimova, S., Grigorian, R., & Razumovskaya, A. (2002). Pineal-regulating tetrapeptide epitalon improves eye retina condition in retinitis pigmentosa. Neuro endocrinology letters, 23(4), 365-368. https://pubmed.ncbi.nlm.nih.gov/12195242/
50. Khavinson, V. K.h, Razumovsky, M. I., Trofimova, S. V., & Razumovskaya, A. M. (2003). Retinoprotective effect of Epithalon in campbell rats of various ages. Bulletin of experimental biology and medicine, 135(5), 495-498. https://doi.org/10.1023/a:1024931812822 https://pubmed.ncbi.nlm.nih.gov/12910293/
51. Khavinson, V. K.h, Zemchikhina, V. N., Trofimova, S. V., & Malinin, V. V. (2003). Effects of peptides on proliferative activity of retinal and pigmented epithelial cells. Bulletin of experimental biology and medicine, 135(6), 597-599. https://doi.org/10.1023/a:1025497806636 https://pubmed.ncbi.nlm.nih.gov/12937684/
52. Khavinson, V. K.h, Razumovskii, M. I., Trofimova, S. V., Grigor'yan, R. A., Khaban, T. V., Oleinik, T. L., & Razumovskaya, A. M. (2002). Effect of epithalon on age-specific changes in the retina in rats with hereditary pigmentary dystrophy. Bulletin of experimental biology and medicine, 133(1), 87-89. https://doi.org/10.1023/a:1015125031829 https://pubmed.ncbi.nlm.nih.gov/12170316/
53. Kossoy, G., Zandbank, J., Tendler, E., Anisimov, V., Khavinson, V., Popovich, I., Zabezhinski, M., Zusman, I., & Ben-Hur, H. (2003). Epithelium and colon carcinogenesis in rats: proliferative activity and apoptosis in colon tumors and mucosa. International journal of molecular medicine, 12(4), 473-477. https://pubmed.ncbi.nlm.nih.gov/12964022/
54. Kossoy, G., Anisimov, V. N., Ben-Hur, H., Kossoy, N., & Zusman, I. (2006). Effect of the synthetic pineal peptide epithelium on spontaneous carcinogenesis in female C3H/He mice. In vivo (Athens, Greece), 20(2), 253-257. https://pubmed.ncbi.nlm.nih.gov/16634527/
55. Anisimov, V. N., Khavinson, V. K.h, Popovich, I. G., & Zabezhinski, M. A. (2002). Inhibitory effect of peptide Epitalon on colon carcinogenesis induced by 1,2-dimethylhydrazine in rats. Cancer letters, 183(1), 1-8. https://doi.org/10.1016/s0304-3835(02)00090-3 https://pubmed.ncbi.nlm.nih.gov/12049808/
56. Anisimov, V. N., Khavinson, V. K., Provinciali, M., Alimova, I. N., Baturin, D. A., Popovich, I. G., Zabezhinski, M. A., Imyanitov, E. N., Mancini, R., & Franceschi, C. (2002). Inhibitory effect of the peptide epitalon on the development of spontaneous mammary tumors in HER-2/neu transgenic mice. International journal of cancer, 101(1), 7-10. https://doi.org/10.1002/ijc.10570 https://pubmed.ncbi.nlm.nih.gov/12209581/
57. Alimova, I. N., Bashurin, D. A., Popovich, I. G., Zabezhinskiĭ, M. A., Volkov, M. A., Provinciali, M., Franceschi, C., Khavincon, B. K.h, & Anisimov, V. N. (2002). Vliianie épitalona i vilona na prodolzhitel'nost' zhizni i razvitie opukholeĭ molochnoĭ zhelezy u samok transgennykh misheĭ erB-2/neu [Effect of Epitalon and Vilon treatment on mammary carcinogenesis in transgenic erbB-2/NEU mice]. Voprosy onkologii, 48(1), 57-60. https://pubmed.ncbi.nlm.nih.gov/12101568/
58. Khavinson, V. K.h, Iuzhakov, V. V., Kvetnoĭ, I. M., & Malinin, V. V. (2001). Vliianie épitalona na kinetiku rosta i funktsional'nuiu morfologiiu sarkomy M-1 [Effect of epithalone on growth kinetics and functional morphology of M-1 sarcoma]. Voprosy onkologii, 47(4), 461-466. https://pubmed.ncbi.nlm.nih.gov/11710291/
59. Anisimov V. N. (2003). The role of pineal gland in breast cancer development. Critical reviews in oncology/hematology, 46(3), 221-234. https://doi.org/10.1016/s1040-8428(03)00021-0 https://pubmed.ncbi.nlm.nih.gov/12791421/
60. Jokhadze, T., Monaselidze, J., Nemsadze, G., Buadze, T., Gaiozishvili, M., & Lezhava, T. (2017). Georgian medical news, (262), 88-92. https://pubmed.ncbi.nlm.nih.gov/28252435/
61. Vinogradova I. A. (2009). Advances in gerontology = Uspekhi gerontologii, 22(4), 631-638. https://pubmed.ncbi.nlm.nih.gov/20405731/
62. Kuznik, B. I., Pateyuk, A. V., Rusaeva, N. S., Baranchugova, L. M., & Obydenko, V. I. (2011). Effects of peptides Lys-Glu-Asp-Gly and Ala-Glu-Asp-Gly on hormonal activity and structure of the thyroid gland in hypophysectomized young chickens and old hens. Bulletin of experimental biology and medicine, 150(4), 495-499. https://doi.org/10.1007/s10517-011-1177-3 https://pubmed.ncbi.nlm.nih.gov/22268052/
63. Kuznik, B. I., Pateyuk, A. V., & Rusaeva, N. S. (2008). Effect of tetrapeptides Lys-Glu-Asp-Gly and Ala-Glu-Asp-Gly on the structure and function of the thyroid gland in neonatally hypophysectomized chickens. Bulletin of experimental biology and medicine, 145(1), 104-107. https://doi.org/10.1007/s10517-008-0033-6 https://pubmed.ncbi.nlm.nih.gov/19024016/
64. Kuznik, B. I., Pateiuk, A. V., Rusaeva, N. S., Baranchugova, L. M., & Obydenko, V. I. (2011). Advances in gerontology = Uspekhi gerontologii, 24(1), 93-98. https://pubmed.ncbi.nlm.nih.gov/21809626/
65. Zamorskiĭ, I. I., & Shchudrova, T. S. (2014). Biofizika, 59(5), 1023-1026. https://pubmed.ncbi.nlm.nih.gov/25730989/
66. Dzhokhadze, T. A., Buadze, T. Z.h, Gaĭozishvili, M. N., Rogava, M. A., & Lazhava, T. A. (2013). Georgian medical news, (225), 94-97. https://pubmed.ncbi.nlm.nih.gov/24423684/
67. Anisimov, S. V., Khavinson, V. K.h, & Anisimov, V. N. (2004). Effect of melatonin and tetrapeptide on gene expression in mouse brain. Bulletin of experimental biology and medicine, 138(5), 504-509. https://doi.org/10.1007/s10517-005-0082-z https://pubmed.ncbi.nlm.nih.gov/15723138/
68. Morozov, A. V., Khizhkin, E. A., Svechkina, E. B., Vinogradova, I. A., Ilyukha, V. A., Anisimov, V. N., & Khavinson, V. K.h (2015). Effects of Geroprotectors on Age-Related Changes in Proteolytic Digestive Enzyme Activities at Different Lighting Conditions. Bulletin of experimental biology and medicine, 159(6), 761-763. https://doi.org/10.1007/s10517-015-3069-4 https://pubmed.ncbi.nlm.nih.gov/26519279/
69. Kozina, L. S., Arutiunian, A. V., Stvolinskiĭ, S. L., & Khavinson, V. K.h (2008). Advances in gerontology = Uspekhi of gerontology, 21(1), 68-73. https://pubmed.ncbi.nlm.nih.gov/18546826/
70. Khavinson, V. K., & Kvetnoii, I. M. (2000). Peptide bioregulators inhibit apoptosis. Bulletin of experimental biology and medicine, 130(12), 1175-1176. https://pubmed.ncbi.nlm.nih.gov/11276315/
71. Yue, X., Liu, S. L., Guo, J. N., Meng, T. G., Zhang, X. R., Li, H. X., Song, C. Y., Wang, Z. B., Schatten, H., Sun, Q. Y., & Guo, X. P. (2022). Epitalone protects against post-ovulatory aging-related damage of mouse oocytes in vitro. Aging, 14(7), 3191-3202. https://doi.org/10.18632/aging.204007 https://pubmed.ncbi.nlm.nih.gov/35413689/
72. Sibarov, D. A., Kovalenko, R. I., Malinin, V. V., & Khavinson, V. K.h (2002). Epithalon influences pineal secretion in stress-exposed rats in the daytime. Neuro endocrinology letters, 23(5-6), 452-454. https://pubmed.ncbi.nlm.nih.gov/12500171/